High-performance liquid chromatographic enantioseparation of beta-amino acids

Direct and indirect high-performance liquid chromatographic methods were de veloped for the enantioseparation of beta -amino acids (beta -substituted- beta -alanines). Direct separation involved the application of chiral colum ns: Crownpak CR(+), Chirobiotic T and Chirobiotic R. Indirect separation wa s based on precolumn derivatization with 2,3,4,6-tetra-O-acetyl-beta -D-glu copyranosyl isothiocyanate or N-alpha-(2,4-dinitro-5-fluorophenyl)-L-alanin eamide (Marfey's reagent), with subsequent separation on an achiral column. The chromatographic conditions were varied to achieve optimum separation. (C) 2001 Elsevier Science B.V. All rights reserved.