H. Dogan et al., Effect of gutta-percha solvents at different temperatures on the calcium, phosphorus and magnesium levels of human root dentin, J ORAL REH, 28(8), 2001, pp. 792-796
The aim of this study in vitro investigation was to evaluate the alteration
s caused by warmed gutta-percha solvents on the calcium, phosphorus and mag
nesium levels of root dentin. Extracted human anterior teeth, whose crowns
and apical root thirds had been removed were used as root dentin specimens.
The roots were sectioned longitudinally into two segments, cleaned and dri
ed. Segments were divided into 12 groups (n = 12). In 6 groups, the specime
ns received treatment with the following solvents at room temperature (22 d
egreesC): Chloroform, xylene, eucalyptol, orange oil, halothane and saline
(control). Within each group, the specimens were further subgrouped into tw
o to be incubated (100% humidity at 37 degreesC) for 5 and 10 min, respecti
vely, following treatment with the solvents. The remaining six groups were
treated with the same solvents which had been previously warmed to body tem
perature (37 degreesC) and received the same experimental procedures. The l
evels of calcium, phosphorus and magnesium in each specimen were analysed u
sing energy dispersive spectrometric microanalysis. Statistical analysis of
the readings showed that neither warming of the solvents nor prolonged inc
ubation (treatment) time was capable of altering the histochemical composit
ion of cut root dentin surfaces.