DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus

We report a further characterization of the genomic region containing the s oybean supernodulation gene NTS-1. We performed a search for new markers li nked to NTS-1 by combining DNA amplification fingerprinting (DAF) and bulke d segregant analysis (BSA). The search resulted in one cloned polymorphism (B44-456) linked in trans, 8.5cM from the locus. Southern hybridization sho wed duplication of the B44-456 sequence in the soybean genome. Additionally , a DNA database search revealed one Arabidopsis thaliana genomic clone fro m chromosome I possessing 62% homology to the B44-456 marker. A relatively low number of polymorphisms were identified by several PCR marker technolog ies for this soybean genomic region, providing an additional support for it s highly conserved and/or duplicated organization.