Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Authors
Gallagher, JA Koroleva, OA Tomos, DA Farrar, JF Pollock, CJ
Citation
Ja. Gallagher et al., Single cell analysis technique for comparison of specific mRNA abundance in plant cells, J PLANT PHY, 158(8), 2001, pp. 1089-1092
Citations number
23
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
JOURNAL OF PLANT PHYSIOLOGY
ISSN journal
01761617 → ACNP
Volume
158
Issue
8
Year of publication
2001
Pages
1089 - 1092
Database
ISI
SICI code
0176-1617(200108)158:8<1089:SCATFC>2.0.ZU;2-E
Abstract
A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose : fructan fructosyltransferas e) was demonstrated in mesophyll and parenchymatous bundle sheath cells. Th is method provides a reliable technique for the analysis of specific gene t ranscript abundance in individual plant cells.