Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

In the previous study, we cloned a new gene, named NGX6, related to nasopha ryngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell li ne HNE1 and that transfected with the gene. Using high-resolution two-dimen sional electrophoresis, we found that 22 protein spots showed variations th at were significant and reproducible. Analysis of matrix-assisted laser des orption/ionization time-of-flight mass spectrometry and database searches i dentified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB , and All receptor-interacting protein (AIP). The functional implications o f the identified proteins are discussed.