Salmon GnRH and its analogues bind the human placental receptor

Citation
Tm. Siler-khodr et M. Grayson, Salmon GnRH and its analogues bind the human placental receptor, J SOC GYN I, 8(4), 2001, pp. 233-238
Citations number
57
Categorie Soggetti
Reproductive Medicine
Journal title
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
ISSN journal
10715576 → ACNP
Volume
8
Issue
4
Year of publication
2001
Pages
233 - 238
Database
ISI
SICI code
1071-5576(200107/08)8:4<233:SGAIAB>2.0.ZU;2-Q
Abstract
Objective: The presence of GnRH receptors in the human placenta has been re cognized for a number of years. However, mammalian GnRH, which is expressed in placental tissues, has limited affinity for the chorionic receptor. On the basis of immunological and bioactivity data, we have previously propose d that the chorionic GnRH may differ from mammalian GnRH. Methods: we have studied the affinity of another isoform of GnRH (ie, salmo n GnRH and stable analogues of this GnRH isoform), and compared their recep tor affinity to that of mammalian GnRH and it analogues. Results: Using our receptor assay method with the labeled mammalian GnRH an alogue Buserelin, salmon GnRH had a twofold greater affinity for the placen tal GnRH receptor than did mammalian GnRH and for the stable salmon GnRH an alogue the affinity was increased tenfold. Using a homologous receptor assa y method with a stable salmon GnRH analogue as label, the affinity for this salmon Gn RH analogue had a K-d of 101 nmol/L. Conclusion: The presence of these higher affinity receptors for non mammali an GnRH in the human placenta has led us to propose that the chorionic tiss ues may express more than on iso form of GnRH and that non-mammalian GnRH, such as salmon GnRH, may be potent regulators of placental functions. (J So c Gynecol Investig 2001; 8:233-8) Copyright (C) 2001 by the Society of Gyne cologic Investigation.