PRODUCTION OF MONOCLONAL-ANTIBODIES TO HUMAN PLACENTAL GLUTATHIONE-S-TRANSFERASE AND ITS PRELIMINARY APPLICATION IN COLONIC-CARCINOMA

Human placental glutathione S-transferase (GST-pi) free of human IgG w as purified by improved method. Four monoclonal antibodies (MoAbs) aga inst GST-pi were obtained by the fusion of murine myeloma cell Sp2/0 w ith spleen cells from BALB/c mice immunized with GST-pi. All of four M oAbs reacted only with GST-pi, not with goat GST, rat GST, human GST-a lpha, GST-mu, IgG and glutahione reductase, when assayed by ELISA and Western blot. ELISA additivity test showed that four MoAbs recognized two groups of different epitopes. Immunohistochemical staining with Mo Abs indicated that GST-pi was a useful tumor marker of colonic carcino ma.