Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using single-strand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences
Ka. Kristiansen et al., Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using single-strand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences, MOL ECOL, 10(8), 2001, pp. 2089-2093
The mitochondrial ribosomal large subunit (Ls) DNA was used to identify the
orchid mycorrhizal fungi found in roots of Dactylorhiza majalis. The gene
was amplified using DNA extracted from single pelotons obtained from fresh
and silica gel dried roots. Furthermore, sequencing a variety of well-chara
cterized orchid isolates expanded the fungal database of the mitochondrial
ribosomal LsDNA. Polymerase chain reaction product length variants present
in D. majalis were sequenced and identified using the expanded database. Th
ese analyses revealed two different peloton-forming fungi in samples from D
. majalis, which sometimes occurred together as a single two-taxa peloton w
ithin the same cortex cell. The first taxon belonged to the genus Tulasnell
a and the second taxon was distantly related to Laccaria.