Carbohydrate-ethanol transition in cereal grains under anoxia

Cereal grains differ greatly in their reponses to anaerobiosis. Here, the i n vivo conversion of carbohydrates to ethanol and CO2 under anoxia is repor ted for three cereal grains. The conversion of glucose, fructose or sucrose to ethanol under anaerobic c onditions was investigated in rice (Oryza sativa), barley (Hordeum vulgare) and wheat (Triticum aestivum) grains; alcohol dehydrogenase (EC 1.1.1.1) a nd pyruvate decarboxylase (EC 4.1.1.1) activities were also analysed under aerobic and anaerobic incubation. Our data suggest that rice grains are able to produce ethanol under anoxia for the whole period of anoxic treatment, whereas barley and wheat grains c an produce this terminal product of fermentation only during the first days of anaerobiosis. The level of enzymes involved in the fermentation pathway increases strongly under anoxic conditions in all three cereals. Conversion of hexose to CO2 is nearly unaffected by anoxia in wheat, barley and rice, whereas only rice grains are able to degrade and utilize sucrose efficiently under anoxia. By contrast, wheat and barley do not utilize suc rose efficiently under anaerobic conditions. (C) New Phytologist (2001).