Synthesis of a new 5 '-O-triphosphate analog of 5-methyl 2 '-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA

Authors
Rodriguez-Tanty, C Perez, R Miranda, J Velez-Castro, H Rosado, A Macias, A Vidal-Alles, V Arana, MJ Souto, NP Higginson-Clarke, D Riveron, AM
Citation
C. Rodriguez-tanty et al., Synthesis of a new 5 '-O-triphosphate analog of 5-methyl 2 '-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA, NUCLEOS NUC, 20(8), 2001, pp. 1449-1461
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
8
Year of publication
2001
Pages
1449 - 1461
Database
ISI
SICI code
1525-7770(2001)20:8<1449:SOAN5'>2.0.ZU;2-N
Abstract
We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenz amido)hex-1-yl]-2 ' -O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-{n-[6-(p-bromobenzamido) caproyl ami no]alk-1-yl}-2 ' -deoxycytidine compounds, and confirmed their presence by H-1-NMR studies. In vitro DNA labeling with modified nucleotides is prelimi narily evaluated.