PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis

Authors
Efimov, V Choob, M Buryakova, A Phelan, D Chakhmakhcheva, O
Citation
V. Efimov et al., PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis, NUCLEOS NUC, 20(4-7), 2001, pp. 419-428
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
419 - 428
Database
ISI
SICI code
1525-7770(2001)20:4-7<419:POMATE>2.0.ZU;2-O
Abstract
DNA mimics containing phosphonate analogues of PNAs (pPNAs), particularly P NA-pPNA hybrids as well as hetero-oligomers consisted of pPNA units and PNA -like molecules on the base of trans-4-hydroxy-L-proline (HypNA) have been synthesized. The evaluation of their effectiveness, in assays based on the hybridization technique in the comparison with natural oligonucleotides and classical PNAs has shown a high potential of these mimics as sensor molecu les for nucleic acid based diagnostics and as molecular probes for mRNA iso lation.