Properties of arabinonucleic acids (ANA & 20 ' F-ANA): Implications for the design of antisense therapeutics that invoke RNase H cleavage of RNA

Authors
Damha, MJ Noronha, AM Wilds, CJ Trempe, JF Denisov, A Pon, RT Gehring, K
Citation
Mj. Damha et al., Properties of arabinonucleic acids (ANA & 20 ' F-ANA): Implications for the design of antisense therapeutics that invoke RNase H cleavage of RNA, NUCLEOS NUC, 20(4-7), 2001, pp. 429-440
Citations number
46
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
429 - 440
Database
ISI
SICI code
1525-7770(2001)20:4-7<429:POAA(&>2.0.ZU;2-V
Abstract
Inversion of configuration of the C2' position of RNA leads to a very uniqu e nucleic acid structure: arabinonucleic acid (ANA). ANA, and its 2'-fluoro derivative (2'F-ANA) form hybrids with RNA that are capable of activating RNase H, resulting in cleavage of the RNA strand. In this paper, we review the properties of duplexes formed between ANA (or 2'F-ANA) and its RNA comp lement. These studies support the notion that RNase H is sensitive to the m inor groove dimensions of the hybrid substrate.