Inhibition of (cytosine C5)-methyltransferase by oligonucleotides containing flexible (cyclopentane) and conformationally constrained (bicyclo[3.1.0]hexane) abasic sites

Authors
Marquez, VE Wang, PY Nicklaus, MC Maier, M Manoharan, M Christman, JK Banavali, NK Mackerell, AD
Citation
Ve. Marquez et al., Inhibition of (cytosine C5)-methyltransferase by oligonucleotides containing flexible (cyclopentane) and conformationally constrained (bicyclo[3.1.0]hexane) abasic sites, NUCLEOS NUC, 20(4-7), 2001, pp. 451-459
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
451 - 459
Database
ISI
SICI code
1525-7770(2001)20:4-7<451:IO(CBO>2.0.ZU;2-W
Abstract
Pseudorotationally locked sugar analogues based on bicyclo[3.1.0]-hexane te mplates were placed in DNA duplexes as abasic target sites in the M.HhaI re cognition sequence. The binding affinity of the enzyme increases when the a basic site is constrained to the South conformation and decreases when it i s constrained to the North conformation. A structural understanding of thes e differences is provided.