Citation
N. Polushin et al., 2 '-modified oligonucleotides from methoxyoxalamido and succinimido precursors: Synthesis, properties, and applications, NUCLEOS NUC, 20(4-7), 2001, pp. 507-514
Citations number
2
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770
→ ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
507 - 514
Database
ISI
SICI code
1525-7770(2001)20:4-7<507:2'OFMA>2.0.ZU;2-E
Abstract
Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) an
d 2'-succinimido (SUC) precursors is described. Their physical and biochemi
cal properties were assessed. Synthesized oligonucleotides were used as pri
mers in advanced DNA sequencing protocols. An example of sequencing directl
y off genomic DNA template without prior cloning or PCR amplification is pr
esented.