High capacity substrates as a platform for a DNA probe array genotyping assay

Authors
Fidanza, J Glazer, M Mutnick, D McGall, G Frank, C
Citation
J. Fidanza et al., High capacity substrates as a platform for a DNA probe array genotyping assay, NUCLEOS NUC, 20(4-7), 2001, pp. 533-538
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
533 - 538
Database
ISI
SICI code
1525-7770(2001)20:4-7<533:HCSAAP>2.0.ZU;2-A
Abstract
Colloidal silica particles were deposited on a glass substrate to produce h igh-capacity porous supports for high-density DNA probe arrays. Porous surf aces were used to increase the addressable surface area and number of probe s available for hybridization. Surfaces derived from 70-100 nm size particl es deposited in films from 0.15 to 2 microns thick exhibited excellent perf ormance in light-directed oligonucleotide synthesis. Evaluation of these su bstrates in a genotyping assay is reported.