A case study: Oligonucleotide purification from gram to hundred gram scale

Authors
Deshmukh, RR Eriksson, KO Moore, P Cole, DL Sanghvi, YS
Citation
Rr. Deshmukh et al., A case study: Oligonucleotide purification from gram to hundred gram scale, NUCLEOS NUC, 20(4-7), 2001, pp. 567-576
Citations number
7
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
567 - 576
Database
ISI
SICI code
1525-7770(2001)20:4-7<567:ACSOPF>2.0.ZU;2-E
Abstract
This article describes the purification and scale-up of ISIS 2302, a 20-mer phosphorothioate oligonucleotide by anion-exchange (AX) chromatography. Th e key operating parameters were optimized at gram scale and further scaled up to hundred gram. SOURCE 30Q, a high efficiency polymeric chromatographic media was used for both the small and large-scale work. High length-based purity and yield were maintained at scale-up. This purification is one of t he largest demonstrations of AX purification of phosphorothioate oligonucle otide.