Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity

Authors
Wang, J Verbeure, B Luyten, I Froeyen, M Hendrix, C Rosemeyer, H Seela, F Van Aerschot, A Herdewijn, P
Citation
J. Wang et al., Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity, NUCLEOS NUC, 20(4-7), 2001, pp. 785-788
Citations number
5
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
785 - 788
Database
ISI
SICI code
1525-7770(2001)20:4-7<785:CNA(FS>2.0.ZU;2-0
Abstract
Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporam idite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable aga inst degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RN ase H, resulting in cleavage of the RNA strand.