Synthesis of oligonucleoside boranophosphates via an H-phosphonate method without nucleobase protection

Authors
Sergueev, DS Sergueeva, ZA Shaw, BR
Citation
Ds. Sergueev et al., Synthesis of oligonucleoside boranophosphates via an H-phosphonate method without nucleobase protection, NUCLEOS NUC, 20(4-7), 2001, pp. 789-795
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
789 - 795
Database
ISI
SICI code
1525-7770(2001)20:4-7<789:SOOBVA>2.0.ZU;2-2
Abstract
Short oligonucleoside boranophosphates containing all four nucleosides were synthesized on solid support using base-unprotected nucleoside H-phosphona te monomers. This strategy avoided irreversible base modifications during t he boronation procedure. Structures of the boranophosphate oligomers were c onfirmed by H-1, P-31, B-10 NMR and MS analysis as well as by enzymatic hyd rolysis.