Polyimidazole conjugated oligonucleotides reach the nucleus of HeLa cells

Authors
Morvan, F Castex, C Vives, E Imbach, JL
Citation
F. Morvan et al., Polyimidazole conjugated oligonucleotides reach the nucleus of HeLa cells, NUCLEOS NUC, 20(4-7), 2001, pp. 805-808
Citations number
4
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
805 - 808
Database
ISI
SICI code
1525-7770(2001)20:4-7<805:PCORTN>2.0.ZU;2-K
Abstract
Oligonucleotide models bearing 6, 12 or 18 histamine residues were synthesi zed on solid support and labeled with fluorescein. Only the oligo with 6 hi stamine residues showed a high uptake in HeLa cells with a nuclear localiza tion. Experiment a 4 degreesC or with bafilomicyn A(1) suggest that uptake proceeded by an endocytosis mechanism followed by a destabilization of the membrane. Once in the cytoplasm the oligo reached rapidly the nucleus.