Interaction of LNA oligonucleotides with MDR1 promoter

Authors
Chernolovskaya, EL Koshkin, AA Vlassov, VV
Citation
El. Chernolovskaya et al., Interaction of LNA oligonucleotides with MDR1 promoter, NUCLEOS NUC, 20(4-7), 2001, pp. 847-850
Citations number
2
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
847 - 850
Database
ISI
SICI code
1525-7770(2001)20:4-7<847:IOLOWM>2.0.ZU;2-Q
Abstract
LNA oligonucleotides [1] can be used for targetting to double stranded DNA by the "strand invasion" mechanism. We used affinity modification by reacti ve oligonucleotide conjugates for investigation of oligonucleotides interac tion with structured DNA. The tested LNAs and oligonucleotides of the same sequence were assayed as anti-mdr1 drugs in different cell cultures. One of the oligos, LNA79 strongly inhibited mdr1 induction in Hela cells and tota lly prevented activation of indr1 in K-562.