Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides

Authors
Carriero, S Braich, RS Hudson, RHE Anglin, D Friesen, JD Damha, MJ
Citation
S. Carriero et al., Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides, NUCLEOS NUC, 20(4-7), 2001, pp. 873-877
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
873 - 877
Database
ISI
SICI code
1525-7770(2001)20:4-7<873:IOIVPS>2.0.ZU;2-T
Abstract
A series of V- and Y-shaped nucleic acids, related to the splicing intermed iates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mR NA splicing in yeast were studied. The exogenous bNAs each effect the effic iency of splicing, yet to different degrees, depending on the sugar composi tion and topology of the molecules. Y-shaped RNAs inhibited the formation o f mRNA (i.e. RNA splicing) to the greatest extent.