Novel propargylamine-linked nucleosides for high throughput SNP genotypingby MALDI-TOF MS

Authors
Wenzel, T Frohlich, T Strassburger, K Richter, S Bimmler, J Franke, C Thomas, I Kostrzewa, M
Citation
T. Wenzel et al., Novel propargylamine-linked nucleosides for high throughput SNP genotypingby MALDI-TOF MS, NUCLEOS NUC, 20(4-7), 2001, pp. 883-887
Citations number
6
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
883 - 887
Database
ISI
SICI code
1525-7770(2001)20:4-7<883:NPNFHT>2.0.ZU;2-T
Abstract
The synthesis of positively charged and mass tagged nucleosides containing a quaternary ammonium functionality within the penultimate position of a pr imer is described. Neutralization of the sugar/thiophosphate backbone by al kylation increases the detection sensitivity in the mass spectrometric anal ysis by a factor of at least 100. The variable introduction of these novel compounds within the extension primers enables flexible design of multiplex genotyping reactions.