The use of (oligonucleotide-maleic anhydride copolymer) conjugates in nucleic acid diagnostic assays: Effect of the number of oligonucleotides per polymer on test sensitivity improvement

Authors
Minard-Basquin, C Chaix, C Pichot, C
Citation
C. Minard-basquin et al., The use of (oligonucleotide-maleic anhydride copolymer) conjugates in nucleic acid diagnostic assays: Effect of the number of oligonucleotides per polymer on test sensitivity improvement, NUCLEOS NUC, 20(4-7), 2001, pp. 895-899
Citations number
6
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
895 - 899
Database
ISI
SICI code
1525-7770(2001)20:4-7<895:TUO(AC>2.0.ZU;2-Y
Abstract
(Polymer-oligonucleotide) conjugates were obtained via direct ON synthesis from the poly(ethylene-alt-maleic anhydride: PEMA) grafted onto glass bead surfaces. The effect of the ON number per polymer chain on binding was eval uated through Tnt experiments. Results were correlated with ELOSA (Enzyme L inked Oligosorbent Assay) tests run with conjugates in the capture step.