5-(Phenylthiomethyl)-2 '-deoxyuridine as an efficient photoreactive precursor to generate single and multiple lesions within DNA fragments

Authors
Bellon, S Gasparutto, D Romieu, A Cadet, J
Citation
S. Bellon et al., 5-(Phenylthiomethyl)-2 '-deoxyuridine as an efficient photoreactive precursor to generate single and multiple lesions within DNA fragments, NUCLEOS NUC, 20(4-7), 2001, pp. 967-971
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
967 - 971
Database
ISI
SICI code
1525-7770(2001)20:4-7<967:5'AAEP>2.0.ZU;2-I
Abstract
5-(Phenylthiomethyl)-2'-deoxyuridine was successfully incorporated into DNA oligomers by automated DNA synthesis using phosphoramidite chemistry. UV e xposure of the latter thionucleoside containing oligonucleotides under anae robic and aerobic conditions gives rise to specific base lesions. The photo products have been isolated and further characterized on the basis of NMR a nd mass spectrometric analyses.