Chimeric RNA with modified backbones: Alternating methylene(methylimino) linked phosphodiester backbone oligonucleotides with 2 '-OH and 2 '-OME groups

Authors
Prhavc, M Bhat, B Just, G Cook, PD Manoharan, M
Citation
M. Prhavc et al., Chimeric RNA with modified backbones: Alternating methylene(methylimino) linked phosphodiester backbone oligonucleotides with 2 '-OH and 2 '-OME groups, NUCLEOS NUC, 20(4-7), 2001, pp. 995-997
Citations number
8
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
995 - 997
Database
ISI
SICI code
1525-7770(2001)20:4-7<995:CRWMBA>2.0.ZU;2-O
Abstract
Synthesis of a novel ribo-MMI dimer with 2'-OH and 2'-OMe in 5'- and 3'-nuc leosides, respectively is presented. The synthesis was accomplished by redu ctive coupling of 3'-deoxy-3'-C-formyluridine and 2'-O-methyl-5'-O-methylam inouridine via a thioacetal as the key intermediate for the top part of the dimer. Incorporation of ribo-MMI dimers into oligonucleotides increased bi nding affinity for target RNA.