Promotion of triplex formation by 2 '-O,4 '-C-methylene bridged nucleic acid (2 ',4 '-BNA) modification: Thermodynamic and kinetic studies

Authors
Torigoe, H Obika, S Imanishi, T
Citation
H. Torigoe et al., Promotion of triplex formation by 2 '-O,4 '-C-methylene bridged nucleic acid (2 ',4 '-BNA) modification: Thermodynamic and kinetic studies, NUCLEOS NUC, 20(4-7), 2001, pp. 1235-1238
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
1235 - 1238
Database
ISI
SICI code
1525-7770(2001)20:4-7<1235:POTFB2>2.0.ZU;2-K
Abstract
We analyzed the effect of 2'-O,4'-C-methylene bridged nucleic acid (2',4'-B NA) modification of triplex-forming oligonucleotide (TFO) on pyrimidine mot if triplex formation at neutral pH, a condition where pyrimidine motif trip lexes are unstable. The binding constant of the pyrimidine motif triplex fo rmation at pH 6.8 with 2',4'-BNA modified TFO was about 20 times larger tha n that observed with unmodified TFO. The observed increase in the binding c onstant at neutral pH by the 2',4'-BNA modification resulted from the consi derable decrease in the dissociation rate constant.