Laboratory diagnosis of respiratory diseases: PCR versus serology

Authors
Haaheim, H Vorland, L Gutteberg, TJ
Citation
H. Haaheim et al., Laboratory diagnosis of respiratory diseases: PCR versus serology, NUCLEOS NUC, 20(4-7), 2001, pp. 1255-1258
Citations number
5
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
1255 - 1258
Database
ISI
SICI code
1525-7770(2001)20:4-7<1255:LDORDP>2.0.ZU;2-N
Abstract
We present a comparison between serology and genetic detection of three bac terial pathogens causing lower respiratory tract infection (LRI). We evalua ted serology and PCR for the detection of Mycoplasma pneumoniae, Bordetella pertussis and Chlamydia pneumoniae from 1712 nasopharyngeal and serum samp les. For 856 nasopharyngeal samples, average PCR time was 7.2 days, the par allel serum samples was 13 days. Automated extraction of nucleic acids redu ces average PCR time to 6.7 days.