A novel approach for the solid phase synthesis of DNA-peptide conjugates

The strategy of this study involves automated synthesis of oligonucleotides on a CPG support using standard cyanoethyl phosphoramidite chemistry follo wed by covalent linkage to peptide fragments bearing a free terminal cl-ami no group and residues with protected side chains. Conjugation was formed th rough an alkyldiisocyanate linker. Conjugates were isolated by cleavage fro m the solid support and deprotection in one step.