The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix

Authors
Elghanian, R Xu, Y McGowen, J Seithoff, M Liu, CG Winick, J Fuller, N Ramakrishnan, R Beuhler, A Johnson, T Mazumder, A Brush, CK
Citation
R. Elghanian et al., The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix, NUCLEOS NUC, 20(4-7), 2001, pp. 1371-1375
Citations number
4
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
1371 - 1375
Database
ISI
SICI code
1525-7770(2001)20:4-7<1371:TUAEO2>2.0.ZU;2-Z
Abstract
Photochemical attachment of synthetic oligonucleotides on the three dimensi onal surface of a polyacrylamide based hydrogel was used in the specific de tection of target oligonucleotides. Covalent attachment of the oligonucleot ide to the hydrogel was mediated by the incorporation of a 2+2 photo-attach able functional group in both the hydrogel and the oligonucleotide probe. E xpression and SNP assays were used to evaluate this platform.