A method for synthesis of an artificial ribonuclease

Authors
Astrom, H Stromberg, R
Citation
H. Astrom et R. Stromberg, A method for synthesis of an artificial ribonuclease, NUCLEOS NUC, 20(4-7), 2001, pp. 1385-1388
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
4-7
Year of publication
2001
Pages
1385 - 1388
Database
ISI
SICI code
1525-7770(2001)20:4-7<1385:AMFSOA>2.0.ZU;2-0
Abstract
5-Amino-2,9-dimethyl-1,10-phenanthroline-oligonucleotide conjugates have be en synthesized. A 2'-O-methyl octaribonucleotide carrying a 2'-aminoethoxym ethyl linker in a central position was produced. Reaction of the aminoneocu proine phenyl carbamate with the fully deprotected oligonucleotide in aqueo us solution gave virtually quantitative conversion into the conjugate. Prel iminary cleavage studies in presence of zinc ions show nuclease activity to wards RNA targets.