Homogenous enzymatic synthesis using a thermo-responsive water-soluble polymer support

Authors
Huang, XF Witte, KL Bergbreiter, DE Wong, CH
Citation
Xf. Huang et al., Homogenous enzymatic synthesis using a thermo-responsive water-soluble polymer support, ADV SYNTH C, 343(6-7), 2001, pp. 675-681
Citations number
37
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
ADVANCED SYNTHESIS & CATALYSIS
ISSN journal
16154150 → ACNP
Volume
343
Issue
6-7
Year of publication
2001
Pages
675 - 681
Database
ISI
SICI code
1615-4150(200108)343:6-7<675:HESUAT>2.0.ZU;2-M
Abstract
Several enzymes immobilized on thermoresponsive polyacrylamide polymers are nearly as active as their soluble forms, and can be recovered for reuse af ter gentle heating and precipitation. Carbohydrates attached to these polym ers have been used for enzymatic glycosylation, and the products have been isolated by thermal precipitation followed by release from the polymer, thu s greatly simplifying product purification in water.