Pigment epithelium-derived factor in the vitreous is low in diabetic retinopathy and high in rhegmatogenous retinal detachment

PURPOSE: To report the levels of pigment epithelium-derived factor in the v itreous of patients with diabetic retinopathy, rhegmatogenous retinal detac hment, and idiopathic macular hole. METHODS: Using enzyme,linked immunosorbent as, say, we measured the levels of pigment epithelium-derived factor in the vitreous of 34 eyes of 33 patie nts who underwent vitrectomy for the treatment of diabetic retinopathy (17 eyes of 16 patients), rhegmatogenous retinal detachment (10 eyes), and idio pathic macular hole (seven eyes). RESULTS: The vitreal concentration of pigment epithelium-derived factor was 1.15 +/- 0.23 mug/ml (mean +/- standard error) in eyes with diabetic retin opathy, 3.28 +/- 0.69 mug/ml in rhegmatogenous retinal detachment, and 1.71 +/- 0.39 mug/ml in idiopathic macular hole. The pigment epithelium-derived factor level in rhegmatogenous retinal detachment was significantly higher than that in diabetic retinopathy (P = .0008) and idiopathic macular hole (P = .034). For eyes with diabetic retinopathy, the pigment epithelium-deri ved factor level was 0.88 +/- 0.21 mug/ml in proliferative diabetic retinop athy and 2.43 +/- 0.37 mug/ml in nonproliferative diabetic retinopathy (P = .0083). Additionally, the pigment epithelium-derived factor level in activ e diabetic retinopathy (0.70 +/- 0.22 mug/ml) was significantly lower than the level in inactive diabetic retinopathy (1.79 +/- 0.35 mug/ml; P = .018) . CONCLUSIONS: These results suggest that pigment epithelium-derived factor i nhibits angiogenesis and that lower levels of pigment epithelium-derived fa ctor may be related to the angiogenesis in diabetic retinopathy and result in active proliferative diabetic retinopathy. The results also suggest that higher levels of pigment epithelium-derived factor in the eyes with rhegma togenous retinal detachment may act as a neuroprotective agent for the deta ched retina. (Am J Ophthalmol 2001;132: 378-382. (C) 2001 by Elsevier Scien ce Inc. All rights reserved.).