Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system - Application to the determination of glucose in serum samples
Av. Lapierre et al., Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system - Application to the determination of glucose in serum samples, ANALYT CHIM, 443(1), 2001, pp. 17-24
A recently introduced redox osmium complex of the type [Os(bpy)(2)CI(pyX](), where pyX corresponds to a pyridine derivative bearing an aldehyde group
, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glass
y carbon electrode surface (upper cell body). This approach has been design
ed in a sealed cell to be used under continuous-flow/stopped-flow/continuou
s-flow processing. The operating characteristic of the integrated bioreacto
r/detector unit has been utilized in the determination of glucose in serum
samples using the electroactive polymer as mediator in the re-oxidation of
GOx and amperometric monitoring. The determinations are based on the rate o
f response tinder stopped-flow conditions. The trend in values of the appar
ent Michaelis-Menten constant confirms the validity of the approach to sens
itive determination utilizing very small amounts of biocatalysts. The deter
mination of glucose was possible with a limit of detection of 0.020 mM in t
he processing of as many as 30 samples per hour. (C) 2001 Elsevier Science
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