Effects of oestradiol and the oestrogen antagonist Ici 182,780 on the delayed type hypersensitivity (DTH) index and on serum levels of IgM and IgG inovariectomised Balb/C and MRL/Mp-Lpr/Lpr mice, a model of systemic lupus erythematosus (SLE)

The effects of oestradiol and the oestrogen receptor antagonist ICI 182,780 were investigated on the DTH index, serum IgG and IgM levels and spleen we ight in female BALB/c and MRL/MP-lpr/lpr mice. At six weeks, the mice were ovariectomised, and one week later, over a four-week period, given biweekly s.c. doses of (i) 5 mul of olive oil, or (ii) 5 mul of oil containing 3.2 mug of 17 beta -oestradiol (E2), or (iii) 5 mul of oil containing (3.2 mul of E2 + ICI 182,780, at a dose of 30 mg/kg), or (iv) the same dose of ICI 1 82,780 alone. E2 significantly raised the DTH index in BALB/c mice; this ef fect was prevented if ICI 182,780 was included in the injection. The DTH in dex in MRL mice was unaffected by any of the treatments. All steroid treatm ents raised serum IgG levels in BALB/c mice, but those in sera of MRL were unaffected and were significantly higher than those measured in BALB/c mice . ICI 182,780 depressed IgM in BALB/c mice, while all steroid treatments in creased IgM in MRL mice. ICI 182,780 depressed spleen weights in both strai ns. Oestrogen may influence B cell function through ICI 182,780-sensitive r eceptors, and ICI 182,780 may have agonist actions on the immune system. (2 00 words)