J. Kaminska et al., Neutrophils promote the release of alpha-6-fucosyltransferase from blood platelets through the action of cathepsin G and elastase, BIOCHIMIE, 83(8), 2001, pp. 739-742
Human blood platelets release alpha -6-fucosyltransferase during coagulatio
n of blood or after stimulation with thrombin or other agonists that cause
platelet activation (Antoniewicz et al., FEBS Lett. 244 (1989) 388-390). Ho
wever, in the absence of neutrophils the thrombin-stimulated platelets rele
ase only a small fraction of alpha -6-fucosyltransferase activity (Kosciela
k et al., Acta Biochim. Polon. 42 (1995) 35-40). We show that the effect of
neutrophils is reproduced by cathepsin G or (less efficiently) by elastase
, the two enzymes that are released by neutrophils during coagulation of bl
ood. We have also localized alpha -6-fucosyltransferase to membrane and alp
ha -granule fractions of platelets that had been disrupted by nitrogen cavi
tation. It is concluded that thrombin-activated neutrophils release catheps
in G and elastase that promote degranulation of platelets and hence the sec
retion of alpha -6-fucosyltransferase. (C) 2001 Societe francaise de biochi
mie et biologie moleculaire/Editions scientifiques et medicales Elsevier SA
S. All rights reserved.