Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

Fluorogenic and fluorescent labeling reagents having a benzofurazan (2,1,3- benzoxadiazole) skeleton such as 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD -F), 4-NN-dimethylan-linosulfonyl-7-fluoro-2,1.3-benzoxadiazole (DBD-F), 4- aminosulfonyl-7fluoro-2,1,3-benzoxadiazole (ABD-F), ammonium 7-fluoro-2,1,3 -benzoxadiazole-4-sulfonate (SBD-F), 4-hydrazino-7-nitro-2,1,3,benzoxadiazo le (NBD-H), 4-NN-dimethylaminosulfonyl-7-hydrazino-2,1,3-benzoxadiazole (DB D-H), 4-nitro-7-N-piperazino2,1,3-benzoxadiazole (NBD-PZ), 4-NN-dimethylami nosulfonyl-7-N-piperazino-2,1,3-benzoxadiazole (DBD-PZ), 4-(N-chloroformylm ethyl-N-methyl)amino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole (DBD- COCI) and 7-NN-dimethylaniinosulfonyl4-(2,1,3-benzoxadiazolyl) isothiocyana te (DBD-NCS) are reviewed in terms of synthetic method, reactivity, fluores cence characteristics. sensitivity and application to analytes. Copyright ( C) 2001 John Wiley & Sons, Ltd.