Thiazole orange as the fluorescent intercalator in a high resolution FID assay for determining DNA binding affinity and sequence selectivity of smallmolecules

Authors
Boger, DL Tse, WC
Citation
Dl. Boger et Wc. Tse, Thiazole orange as the fluorescent intercalator in a high resolution FID assay for determining DNA binding affinity and sequence selectivity of smallmolecules, BIO MED CH, 9(9), 2001, pp. 2511-2518
Citations number
65
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY
ISSN journal
09680896 → ACNP
Volume
9
Issue
9
Year of publication
2001
Pages
2511 - 2518
Database
ISI
SICI code
0968-0896(200109)9:9<2511:TOATFI>2.0.ZU;2-2
Abstract
The viability of using thiazole orange as an alternative to ethidium bromid e in a fluorescent intercalator displacement (FID) assay is explored by pro filing the DNA binding affinity and sequence selectivity of netropsin. Util izing a library of hairpin deoxyoligonucleotides containing all possible fo ur base-pair sequences, the method provides a high resolution profile of th e DNA binding properties of small molecules in a high throughput format. (C ) 2001 Elsevier Science Ltd. All rights reserved.