Chemo-enzymatic synthesis of the Galili epitope Gal alpha(1 -> 3)Gal beta(1 -> 4)GlcNAc on a homogeneously soluble PEG polymer by a multi-enzyme system

Authors
Brinkmann, N Malissard, M Ramuz, M Romer, U Schumacher, T Berger, EG Elling, L Wandrey, C Liese, A
Citation
N. Brinkmann et al., Chemo-enzymatic synthesis of the Galili epitope Gal alpha(1 -> 3)Gal beta(1 -> 4)GlcNAc on a homogeneously soluble PEG polymer by a multi-enzyme system, BIOORG MED, 11(18), 2001, pp. 2503-2506
Citations number
21
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
11
Issue
18
Year of publication
2001
Pages
2503 - 2506
Database
ISI
SICI code
0960-894X(20010917)11:18<2503:CSOTGE>2.0.ZU;2-Y
Abstract
The alpha -Gal trisaccharide Gal alpha (1 -->3)Gal beta (1 -->4)GlcNAc 11 w as synthesized on a homogeneously soluble polymeric support (polyethylene g lycol, PEG) by use of a multi-enzyme system consisting of beta -1,4-galacto syltransferase (EC 2.4.1.38), alpha -1,3-galactosyltransferase (EC 2.4.1.15 1), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2) . In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free alp ha -Gal trisaccharide. (C) 2001 Elsevier Science Ltd. All rights reserved.