Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzymeA

Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependen t oxygenase, catalyses an essential step in the mammalian metabolism of bet a -methylated fatty acids. Phytanoyl-CoA was synthesised and used to develo p in vitro assays for PAHX. The product of the reaction was confirmed as 2- hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully acti ve. (C) 2001 Elsevier Science Ltd. All rights reserved.