Extrusion of actin-positive strands from HEp-2 and Int 407 cells caused byouter membrane preparations of enteropathogenic Escherichia coli and specific attachment of wild type bacteria to the strands
Ss. Kumar et al., Extrusion of actin-positive strands from HEp-2 and Int 407 cells caused byouter membrane preparations of enteropathogenic Escherichia coli and specific attachment of wild type bacteria to the strands, CAN J MICRO, 47(8), 2001, pp. 727-734
Enteropathogenic Escherichia coli (EPEC) causes persistent infantile diarrh
oea. This nontoxigenic E. coli exhibits a complicated pathogenic mechanism
in which its outer membrane proteins and type III secretory proteins damage
intestinal epithelium and cause diarrhoea. In accordance with this, our pr
evious study using HEp-2 cells demonstrated cytopathic effects caused by ce
ll-free outer membrane preparations of EPEC. In this study, we report the e
xtrusion of actin-positive strands from HEp-2 and Int 407 cells when treate
d with outer membrane preparations. An interesting observation of this work
, perhaps relevant to the characteristic localized three-dimensional colony
formation of EPEC, is the attachment of a wild type EPEC strain to these a
ctin-positive strands.