BNM1, A BRASSICA POLLEN-SPECIFIC GENE

cDNA and genomic clones of a new pollen-specific gene, Bnm1, have been isolated from Brassica napus cv. Topas. The gene contains an open rea ding frame of 546 bp and a single intron of 362 bp. A comparison of th e deduced amino acid sequence with sequences in data banks did not sho w similarity with known proteins. Northern blot analysis of developing pollen showed that Bnm1 mRNA was first detected in bicellular pollen and accumulated to higher levels in tricellular pollen. Bnm1 mRNA was not detected in leaves, stems, roots, pistils, seeds or pollen-derived embryos. RNA in situ hybridization of whole flower buds confirmed tha t Bnm1 was pollen-specific and expressed late in development. A promot er fragment of the Bnm1 gene fused to the gusA reporter gene yielded s imilar patterns of tissue specificity and developmental regulation in transgenic B. napus cv. Westar plants; however, the promoter was also active during the early stages of pollen development. The Bnm1 gene, c loned in this study, was derived from the A genome of the allotetraplo id species B. napus (AACC). Southern blot analysis indicated that sequ ences similar to the Bnm1 gene were found in both A and C Brassica gen omes. Related sequences were found in all 10 members of the Brassiceae tribe examined, but were not present in all tribes of the Brassicacea e family.