A COMPLETE YAC CONTIG AND COSMID INTERVAL MAP COVERING THE ENTIRETY OF HUMAN XQ21.33 TO XQ22.3 FROM DXS3 TO DXS287

We have produced a physical map that covers the entirety of Xq21.33 to Xq22.3, from DXS3 to DXS287, approximately 15-17 Mb of the proximal l ong arm of the human X chromosome. This region has already been shown to contain a number of genes involved in genetic disorders, some of wh ich have yet to be cloned. The physical map consists of a contig of 42 0 yeast artificial chromosome (YAC) clones ordered with respect to 142 DNA markers, approximately one probe every 110 kb. Forty-three YACs f rom across the contig have been used to isolate 2019 cosmids that have been mapped into 87 intervals, and 667 of these clones are positive f or at least 1 single-copy marker. These YACs and cosmids have been use d to confirm data from other published contigs that map to the region. The physical map described here constitutes the first step toward a c omplete transcriptional map of the region. (C) 1997 Academic Press.