HIGH-RESOLUTION RECOMBINATIONAL MAP OF MOUSE-CHROMOSOME-16

Five intersubspecific backcrosses and an intercross were used to estab lish a sex-averaged recombinational map spanning 56 cM across most of mouse Chromosome 16 (Chr 16). A total of 123 markers were ordered usin g an interval mapping approach to identify 425 recombination sites in a collection of 1154 meioses from 1155 progeny generated in the six cr osses. The markers include the 10 ''classic'' Chr 16 reference markers , 26 additional genes or transcripts including two phenotypic markers (Pit1(dw) and Kcnj6(wv)), and 87 simple sequence length polymorphisms (SSLPs). One set of monozygotic twins was detected among the 304 meios es mapped to highest resolution. The reference markers and SSLPs allow the map to be well integrated with existing maps of Chr 16. The avera ge distance between crossover sites is less than 500 kb for most chrom osomes, making this collection of recombinant chromosomes useful as a binning and ordering resource for YAC-based physical map assembly on C hr 16. (C) 1997 Academic Press.