Objective. To assess serum free testosterone (free T) concentrations in var
ious groups of women by means of an ultrafiltration technique (UFT) or two
RIAs and to investigate the influence of levonorgestrel (LNG) and norethist
erone (NET) on free T.
Design. Experimental study with serum samples from various groups of patien
ts.
Setting: Obstetrics and gynecology department of a university.
Patient(s): Samples from 20 normal healthy women, 20 menopausal women, 21 p
regnant women, 23 women using oral contraceptives (OC), 20 hyperandrogenemi
c women, 20 hirsute patients with normal total testosterone (total T), 18 p
atients with acne (normal total T) and 9 women with adrenogenital syndrome
(AGS).
Intervention(s): Blood samples were taken by venipuncture.
Main outcome Measure(s): Serum concentrations of free T. Result(s): Mean fr
ee-T levels determined by UFT amounted to 5-6 pg/mL in most groups, except
in patients with hyperandrogenemia (8.3 pg/mL) or AGS (10.7 pg/mL) and in w
omen using OC (1.9 pg/mL). The values were twofold to fourfold and threefol
d to sevenfold higher than those determined with the RIAs. The proportion o
f free T as determined by UFT was 0.9-1.2% of total T and was 0.55% during
OC treatment. The presence of 10 ng/mL of LNG or NET increased free-T level
s by only 13%.
Conclusion(s): Serum free-T levels determined with RIA are much lower than
those measured with the UFT. The ratio between the UFT and RIA values may v
ary depending on the endocrine status. In women with normal or elevated sex
hormone-binding globulin levels, nortestosterone derivatives increase free
T levels only slightly. (Fertil Steril(R) 2001,76:576-82. (C) 2001 by Amer
ican Society for Reproductive Medicine).