Cmgc. Renard et al., Interactions between apple cell walls and native apple polyphenols: quantification and some consequences, INT J BIO M, 29(2), 2001, pp. 115-125
Citations number
41
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Cell walls were prepared from apple parenchyma by a phenol:buffer procedure
. Polyphenols were extracted from freeze-dried apple parenchyma by methanol
and water:acetone, and purified by preparative HPLC. Interactions were qua
ntified by bringing into contact suspended cell walls and polyphenol soluti
ons. Hydroxycinnamic acids and (-)-epicatechin did not bind to cell walls.
Binding of procyanidins was fast and reached up to 0.6 g per g cell walls.
The amounts of procyanidins bound increased with the initial concentration
and with DPn. Procyanidins could be partially desorbed by buffer, more bein
g desorbed in the presence of dissolved cell wall polysaccharides. They wer
e totally desorbed using 8 M urea or acetone:water. Hi.-her polymers were b
ound selectively from procyanidin mixtures, and very high average DPn were
obtained in extensively washed complexes. Binding of procyanidins inhibited
enzymic degradation of the cell walls. (C) 2001 Elsevier Science B.V. All
rights reserved.