Conversion of amino acids into aroma compounds by cell-free extracts of Lactobacillus helveticus

Aims: Lactobacillus helveticus is an essential starter in Swiss-type cheese s such as Emmental. This study was to determine whether cell-free extracts of Lact. helveticus were able to convert free amino acids into neutral vola tile aroma compounds at the pH and temperature occurring in cheese. Methods and Results: A mix of branched-chain (Leu, Ile, Val), aromatic (Tyr , Phe) and sulphur (Met) amino acids was incubated for 7 days, at pH 5.7 an d 24 degreesC, with cell-free extracts of six strains. The amino acids were all transaminated into the corresponding keto acids when an amino group ac ceptor (alpha -ketoglutaric acid) was provided. Phe and Tyr were transamina ted the most efficiently, followed by Leu, Met, Ile and Val. Three major vo latile compounds were detected by GC-MS: benzaldehyde, dimethyl disulphide and 2-methyl propanol. Whatever the strain, benzaldehyde was produced in th e highest quantity (0.25-1 mu mol l(-1) mg(-1) protein). Conclusions, Significance and Impact of the Study: Lactobacillus helveticus intracellular enzymes could significantly contribute to the production of aroma compounds from amino acid catabolism.