Mutation and overexpression of the beta-catenin gene may play an importantrole in primary hepatocellular carcinoma among Chinese people

Aim: To study the role of beta -catenin gene mutation and expression in hep atocellular carcinogenesis. Method. Thirty-four hepatocellular carcinoma (H CC) specimens and adjacent para-cancerous tissues, and four normal liver ti ssues were analyzed. Subcellular distribution of beta -catenin was examined by immunohistochemistry staining. Mutation and semiquantitative expression of beta -catenin gene exon 3 mRNA were detected by RT-PCR-SSCP and in situ hybridization. Result: Immunohistochemistry showed that all normal liver t issues and para-cancerous tissues examined showed membranous-type staining for beta -catenin protein., frequently with weak expression in the cytoplas m, but no beta -catenin accumulation in nuclei was found; while in liver ca ncer, 21 cases (61.8 %) of HCC examined showed accumulated type in cytoplas ms or nuclei. On SSCP, 15 cases (44.1 %) of HCC altogether displayed three kinds of characteristic mutational mobility shifts. No abnormal shifting ba nds were found in tissues from normal liver or para-cancerous area. The bet a -catenin gene exon 3 mRNA expression index of 34 HCCs was higher than tha t of para-cancerous tissue and normal liver tissue. Using in situ hybridiza tion, the signal corresponding to beta -catenin gene exon 3 mRNA was partic ularly strong in cytoplasm of HCC when compared with those of paracancerous tissues and normal liver tissues. Conclusion: beta -catenin gene mutation and overexpression may have a critical role in malignant progression of hep atic carcinogenesis among Chinese people.