Integrin alpha(v)beta(3) is involved in stimulated migration of vascular adventitial fibroblasts by basic fibroblast growth factor but not platelet-derived growth factor

We examined the effects of basic fibroblast growth factor (bFGF) and platel et-derived growth factor (PDGF) on the migration of vascular adventitial fi broblasts (VAFs) isolated from rat aortic adventitiae. Both bFGF and PDGF s ignificantly stimulated VAF migration in vitro. An antibody to rat beta (3) integrin reduced bFGF-stimulated migration in a dose dependent manner. Mor eover, VAF migration was inhibited in the presence of cyclic RGD (cRGD) pep tide. However, PDGF-directed migration was blocked only by equivalent cRGD peptide but not by antibody to beta (3) integrin. These data suggest that a lpha (v)beta (3) integrin mediates VAF migration stimulated by bFGF and tha t chemoattractant directed migration may be through distinct integrins. J. Cell. Biochem. 83: 129-135, 2001, (C) 2001 Wiley-Liss, Inc.