Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

Authors
Edwards, KJ Metherell, LA Yates, M Saunders, NA
Citation
Kj. Edwards et al., Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis, J CLIN MICR, 39(9), 2001, pp. 3350-3352
Citations number
15
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3350 - 3352
Database
ISI
SICI code
0095-1137(200109)39:9<3350:DORMIM>2.0.ZU;2-B
Abstract
A biprobe assay utilizing LightCycler technology was developed to detect ri fampin resistance-associated gene mutations in the Mycobacterium tuberculos is rpoB gene. Three biprobes detected all mutations present in the 46 rifam pin-resistant isolates. Wild-type sequences were correctly identified in ea ch case. The method was reproducible, accurate, and easy to use.