Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR

Authors
Becker, K Badehorn, D Keller, B Schulte, M Bohm, KH Peters, G Fegeler, W
Citation
K. Becker et al., Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR, J CLIN MICR, 39(9), 2001, pp. 3356-3359
Citations number
24
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3356 - 3359
Database
ISI
SICI code
0095-1137(200109)39:9<3356:IACOAS>2.0.ZU;2-4
Abstract
A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gen e fragment was developed by analysis of C. glabrata-specific agarose gel ba nds, which were generated by arbitrarily primed PCR. The expected PCR produ ct was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.